Production and Use of Monoclonal Antibodies for Identification of Strains of Rhizobium trifolii

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Production and Use of Monoclonal Antibodies for Identification of Strains of Rhizobium trifolii.

We produced a monoclonal antibody against Rhizobium trifolii 162x95. This antibody in cell culture supernatant was used in an indirect enzyme-linked immunosorbent assay to differentiate strain 162x95 from naturalized strains in the Appalachian region. Nodules crushed in 0.1 to 0.2 ml of phosphate-buffered saline and used to charge enzyme-linked immunosorbent assay plates gave strong absorbance ...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1986

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.52.1.119-123.1986